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Key Experiments in Practical Developmental Biology - Jennifer Knight

Contents


SECTION I. GRAFTINGS
1. Two developmental gradients control head formation in hydra 4
H. R. BODE
2. Embryonic regulation and induction in sea urchin development 23
C. A. ETTENSOHN
3. The isthmic organizer and brain regionalization in chick embryos 37
D. ECHEVARR´IA and S. MART´INEZ
SECTION II. SPECIFIC CHEMICAL REAGENTS
4. Chemotaxis of aggregating Dictyostelium cells 50
G. GERISCH and M. ECKE
5. Inhibition of signal transduction pathways prevents head regeneration
in hydra 67
L. M. SALGADO

6. Retinoic acid during limb regeneration 77
M. MADEN

SECTION III. BEAD IMPLANTATION
7. Experimental manipulations during limb development in avian embryos 85
Y. GA˜N ´ AN, J. RODR´I GUEZ-LEO´ N, and D. MAC´IAS
8. Induction of ectopic limb outgrowth in chick with FGF-8 99
´ A. RAYA, C. RODR´IGUEZ ESTEBAN, and J. C. IZPISU´ A-BELMONTE
SECTION IV. NUCLEIC ACID INJECTIONS
9. RNAi techniques applied to freshwater planarians (Platyhelminthes)
during regeneration 106
D. BUENO, R. ROMERO, and E. SAL´O
10. Microinjection of Xenopus embryos 117
R. J. GARRIOCK and P. A. KRIEG
SECTION V. GENETIC ANALYSIS
11. Segmental specification in Drosophila melanogaster 127
L. DE NAVAS, M. SUZANNE, D. FORONDA, and E. SA´ NCHEZ-HERRERO
12. Genetic analysis of flower development in Arabidopsis thaliana. The ABC
model of floral organ identity determination 143
J. L. RIECHMANN
13. Genetic analysis of vulva development in C. elegans 153
S. CANEVASCINI
SECTION VI. CLONAL ANALYSIS
14. The role of the gene apterous in the development of the Drosophila wing 167
F. J. D´IAZ-BENJUMEA
15. Extramacrochaetae, an example of a gene required for control of limb size
and cell differentiation during wing morphogenesis in Drosophila 178
A. BAONZA
16. Hedgehog transduction pathway is involved in pattern formation
of Drosophila melanogaster tergites 190
M. MAR´I-BEFFA
SECTION VII. IN SITU HYBRIDIZATION
17. Retinoic acid signalling controls anteroposterior patterning of the
zebrafish hindbrain 205
G. BEGEMANN
18. Left–right asymmetry in the mouse 217
M. BLUM, A. SCHWEICKERT, and C. KARCHER

SECTION VIII. TRANSGENIC ORGANISMS
19. Bicoid and Dorsal: Two transcription factor gradients that specify cell
fates in the early Drosophila embryo 231
S. ROTH
20. Significance of the temporal modulation of Hox gene expression on
segment morphology 255
J. CASTELLI-GAIR HOMBR´IA
21. The UAS/GAL4 system for tissue-specific analysis of EGFR gene function
in Drosophila melanogaster 269
J. B. DUFFY and N. PERRIMON
22. Neurogenesis in Drosophila: A genetic approach 282
C. KLA¨MBT and H. VAESSIN
23. Role of the achaete-scute complex genes in the development of the adult
peripheral nervous system of Drosophila melanogaster 296
S. SOTILLOS and S. CAMPUZANO
SECTION IX. VERTEBRATE CLONING
24. The conservation of the genome and nuclear reprogramming in Xenopus 310
J. B. GURDON
SECTION X. CELL CULTURE
25. In vitro culture and differentiation of mouse embryonic stem cells 316
A. ROLLETSCHEK, C. WIESE, and A. M. WOBUS
SECTION XI. EVO–DEVO STUDIES
26. Microevolution between Drosophila species 330
N. SKAER and P. SIMPSON
SECTION XII. COMPUTATIONAL MODELLING
27. Theories as a tool for understanding the complex network
of molecular interactions 346
H. MEINHARDT

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