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Steroid Receptor Methods - Benjamin A. Lieberman

Contents


PART I. BIOINFORMATICS....................................................................................1
1 Bioinformatics of Nuclear Receptors
Mark Danielsen ....................................................................................... 3
2 Phylogenetic Inference and Parsimony Analysis
Llewellyn D. Densmore III ................................................................... 23
PART II. PURIFICATION PROTOCOLS ..................................................................... 37
3 Expression and Purification of Recombinant Human Progesterone
Receptor in Baculovirus and Bacterial Systems
Vida Senkus Melvin and Dean P. Edwards....................................... 39
4 High-Yield Purification of Functionally Active Glucocorticoid Receptor
Terace M. Fletcher, Barbour S. Warren, Christopher T. Baumann,
and Gordon L. Hager ....................................................................... 55
5 Production and Purification of Histidine-Tagged Dihydrotestosterone-
Bound Full-Length Human Androgen Receptor
Mingmin Liao and Elizabeth M. Wilson ............................................. 67
6 Large Scale Production of Nuclear Receptor Ligand-Binding Domains
Li-Zhi Mi and Fraydoon Rastinejad ................................................... 81
7 Physical Structure of Nuclear Receptor–DNA Complexes
Scott A. Chasse and Fraydoon Rastinejad ...................................... 91
8 Isolation of Steroid-Regulated Genes from the Uterus by mRNA
Differential Display
Sushma Kumar, Maarit Angervo, Milan K. Bagchi,
and Indrani C. Bagchi ................................................................... 105
9 Identification of Nuclear Hormone Receptor Homologs by Screening
Libraries with Highly Degenerate Oligonucleotide Probes
Bruce Blumberg ................................................................................. 119
PART III. STEROID HORMONE-BINDING ASSAYS ................................................... 131
10 Use of [99mTc] Technetium-Labeled Steroids as Probes for
Steroid Hormone Receptors
Frank Wüst.......................................................................................... 133

11 Steroid Hormone Metabolites and Hormone Binding Assays
Rosemary Bland and Martin Hewison............................................. 145
12 Vitamin D3 Analog Screening
Sami Väisänen, Sanna Ryhänen, and Pekka H. Mäenpää ............ 163
PART IV. PROTEIN INTERACTION ASSAYS ........................................................... 177
13 Application of Green Fluorescent Protein to the Study
of Dynamic Protein–Protein Interactions and Subcellular
Trafficking of Steroid Receptors
Steven K. Nordeen, Paul R. Housley, Yihong Wan,
and Richard N. Day ....................................................................... 179
14 Knockout Mice and Steroid Receptor Research
Per Flodby, Stephan Teglund, and Jan-Åke Gustafsson ............. 201
15 Yeast Two-Hybrid Screening for Proteins that Interact with Nuclear
Hormone Receptors
Bertrand Le Douarin, David M. Heery, Claudine Gaudon,
Elmar vom Baur, and Régine Losson ......................................... 227
16 Isolation of a p300/CBP Cointegrator-Associated Protein
Coactivator Complex
Rabindra N. Bhattacharjee, Caroline Underhill,
and Joseph Torchia ...................................................................... 249
17 Nonradioactive Photoaffinity Labeling of Steroid Receptors
Using Western Blot Detection System
Simon J. Evans and Frank L. Moore ............................................... 261
18 Analysis of Steroid Hormone-Induced Histone Acetylation
by Chromatin Immunoprecipitation Assay
James R. Lambert and Steven K. Nordeen .................................... 273
19 Analyzing the Contributions of Chromatin Structure
in Nuclear Hormone Receptor Activated Transcription In Vivo
Christy J. Fryer and Trevor K. Archer............................................. 283
20 Cotransfection Assays and Steroid Receptor Biology
Shimin Zhang and Mark Danielsen ................................................. 297
21 Estrogen Receptor mRNA In Situ Hybridization Using Microprobe
System
Hironobu Sasano, Sachiko Matsuzaki, and Takashi Suzuki ........ 317
PART V. CANCER RESEARCH AND DRUG DISCOVERY ........................................... 327
22 Solid Tumor Cancer Markers and Applications to Steroid Hormone
Research
Marcia V. Fournier, Katherine J. Martin, and Arthur B. Pardee ..... 329

23 Assessing Modulation of Estrogenic Activity of Environmental
and Pharmaceutical Compounds Using MCF-7 Focus Assay
Kathleen F. Arcaro and John F. Gierthy ......................................... 341
24 Combinatorial Chemistry in Steroid Receptor Drug Discovery
John A. Flygare, Daniel P. Sutherlin, and S. David Brown .......... 353
25 Identification of Nuclear Receptor Interacting Proteins Using Yeast
Two-Hybrid Technology: Applications to Drug Discovery
Sunil Nagpal, Corine R. Ghosn,
and Roshantha A.S. Chandraratna ............................................. 359

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